Coding

Part:BBa_K3788011:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-27)

The pelB and sfGFP sequences are made from respectively BBa_J32015 and BBa_I746916. And Aida-I sequence is taking from the UniProt data base with mutation on his coding sequence to get aspartic acid (Substitution D>N).

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.